Rat Tryptase, Tpsab1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TPS,Tpsab1,Tryptase,tryptase alpha/beta 1; tryptase skin; tryptase beta 1; tryptase alpha/beta-1; Mcpt7; TPSB1; rMCP-7; MMCP-7; mast cell protease 7
Search name
Rat TPS ELISA KIT,Rat Tpsab1 ELISA KIT ,Rat Tryptase ELISA KIT ,Rat tryptase alpha/beta 1 ELISA KIT ,Rat tryptase skin ELISA KIT ,Rat tryptase beta 1 ELISA KIT ,Rat tryptase alpha/beta-1 ELISA KIT ,Rat Mcpt7 ELISA KIT ,Rat TPSB1 ELISA KIT ,Rat rMCP-7 ELISA KIT ,Rat MMCP-7 ELISA KIT ,Rat mast cell protease 7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Tryptase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tryptase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tryptase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tryptase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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