Rat Tumor susceptibility gene 101 protein, TSG101 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TSG101,ESCRT-I complex subunit TSG101; TSG10; VPS23; tumor susceptibility gene 10; tumor susceptibility gene 101; tumor susceptibility gene 101 protein; tumor susceptibility protein; tumor susceptibility 101

Search name

Rat TSG101 ELISA KIT ,Rat ESCRT-I complex subunit TSG101 ELISA KIT ,Rat TSG10 ELISA KIT ,Rat VPS23 ELISA KIT ,Rat tumor susceptibility gene 10 ELISA KIT ,Rat tumor susceptibility gene 101 ELISA KIT ,Rat tumor susceptibility gene 101 protein ELISA KIT ,Rat tumor susceptibility protein ELISA KIT ,Rat tumor susceptibility 101 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Tumor susceptibility gene 101 protein ELISA KIT, TSG101 concentrations in serum ELISA KIT, Plasma ELISA KIT, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TSG101. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TSG101 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TSG101 ELISA KIT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TSG101 in the samples is then determined by comparing the O.D. of the samples to the standard curve.