Rat Ubiquitin Carboxyl Terminal Hydrolase L1 ELISA Kit

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  • Model: ELA-E1741r
  • 20 Units in Stock
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Rat Ubiquitin Carboxyl Terminal Hydrolase L1 ELISA Kit

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  UCHL1

Protein Name  UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1

Alternative Name

Rat UCHL1 ELISA KIT ,Rat HEL-117 ELISA KIT ,Rat HEL-S-53 ELISA KIT ,Rat NDGOA ELISA KIT ,Rat PARK5 ELISA KIT ,Rat PGP 9.5 ELISA KIT ,Rat PGP9.5 ELISA KIT ,Rat PGP95 ELISA KIT ,Rat SPG79 ELISA KIT ,Rat Uch-L1 ELISA KIT ,Rat ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA KIT ,Rat epididymis luminal protein 117 ELISA KIT ,Rat epididymis secretory protein Li 53 ELISA KIT ,Rat neuron cytoplasmic protein 9.5 ELISA KIT ,Rat ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) ELISA KIT ,Rat ubiquitin thioesterase L1 ELISA KIT ,Rat ubiquitin thiolesterase ELISA KIT ,Rat ubiquitin C-terminal hydrolase L1 ELISA KIT

 

Intended use

Rat UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 ELISA KIT allows for the in vitro quantitative determination of UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Rat UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 ELISA KIT has been pre-coated with an UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 antibody specific to UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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