Rat Ubiquitin carboxyl- terminal hydrolase BAP1, BAP1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

BAP1,HUCEP-13; UCHL2; hucep-6; cerebral protein 6; cerebral protein-13; BRCA1 associated protein-1

Search name

Rat BAP1 ELISA KIT ,Rat HUCEP-13 ELISA KIT ,Rat UCHL2 ELISA KIT ,Rat hucep-6 ELISA KIT ,Rat cerebral protein 6 ELISA KIT ,Rat cerebral protein-13 ELISA KIT ,Rat BRCA1 associated protein-1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Ubiquitin carboxyl- terminal hydrolase BAP1, BAP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin carboxyl-terminal hydrolase BAP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase BAP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase BAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin carboxyl-terminal hydrolase BAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product

Delivery time 4-6month