Rat UDP- glucuronosyltransferase 2B1, Ugt2b1 ELISA KIT

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  • Model: ELI-28566r
  • 20 Units in Stock
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Rat UGT2B1 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  UGT2B1

Protein Name  UDP-glucuronosyltransferase 2B1

Alternative Name

Rat Ugt2b1 ELISA KIT ,RatUDP-glucuronosyltransferase 2B1 ELISA KIT ,RatUDPGT 2B1 ELISA KIT ,RatUDPGTr-2 ELISA KIT ,RatUdpgtr2 ELISA KIT

Intended use

Rat UGT2B1 ELISA KIT allows for the in vitro quantitative determination of UGT2B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Rat UGT2B1 ELISA KIT has been pre-coated with an UGT2B1 antibody specific to UGT2B1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for UGT2B1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UGT2B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UGT2B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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