Rat Vasoactive intestinal polypeptide receptor 1, VIPR1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

VIPR1,VIP-R-1; HVR1; PACAP-R-2; PACAP-R2; RDC1; V1RG; VAPC1; VIPR; VIRG; VPAC1; VPAC1R; VPCAP1R; PACAP type II receptor; VIP and PACAP receptor 1; VIP receptor, type I; pituitary adenylate cyclase activating polypeptide receptor, type II; vasoactive intestinal polypeptide receptor 1; vasoactive intestinal peptide receptor 1

Search name

Rat VIPR1 ELISA KIT ,Rat VIP-R-1 ELISA KIT ,Rat HVR1 ELISA KIT ,Rat PACAP-R-2 ELISA KIT ,Rat PACAP-R2 ELISA KIT ,Rat RDC1 ELISA KIT ,Rat V1RG ELISA KIT ,Rat VAPC1 ELISA KIT ,Rat VIPR ELISA KIT ,Rat VIRG ELISA KIT ,Rat VPAC1 ELISA KIT ,Rat VPAC1R ELISA KIT ,Rat VPCAP1R ELISA KIT ,Rat PACAP type II receptor ELISA KIT ,Rat VIP and PACAP receptor 1 ELISA KIT ,Rat VIP receptor ELISA KIT ,Rat  type I ELISA KIT ,Rat pituitary adenylate cyclase activating polypeptide receptor ELISA KIT ,Rat  type II ELISA KIT ,Rat vasoactive intestinal polypeptide receptor 1 ELISA KIT ,Rat vasoactive intestinal peptide receptor 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Vasoactive intestinal polypeptide receptor 1, VIP-R-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to VIP-R-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VIP-R-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VIP-R-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VIP-R-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.