Rat Vitamin D3 receptor, VDR ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Vitamin D3 receptor,VDR,1,25-dihydroxyvitamin D3 receptor,Nuclear receptor subfamily 1 group I member 1,
NR1I1,1,25-dihydroxyvitamin D3 receptor,nuclear receptor subfamily 1 group I member 1,vitamin D nuclear receptor variant 1,vitamin D3
receptor
Search name
Rat Vitamin D3 receptor ELISA Kit,Rat VDR ELISA Kit,Vitamin D3 receptor ELISA Kit, VDR ELISA Kit,ELISA Kit, Vitamin
D3 receptor,VDR,1,25-dihydroxyvitamin D3 receptor,Nuclear receptor subfamily 1 group I member 1,
NR1I1,1,25-dihydroxyvitamin D3 receptor,nuclear receptor subfamily 1 group I member 1,vitamin D nuclear receptor variant
1,vitamin D3 receptor
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat vitamin d3 receptor,VDR concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDR. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VDR and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain VDR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VDR in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
