Rat von Willebrand factor, vWF ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
von Willebrand factor(vWF),; von Willebrand factor,F8VWF; VWD; coagulation factor VIII VWF

Search name
Rat von Willebrand factor ELISA Kit, Rat vWF ELISA Kit, von Willebrand factor ELISA Kit, vWF ELISA Kit, Rat F8VWF ELISA Kit,
Rat VWD ELISA Kit, Rat coagulation factor VIII VWF ELISA Kit, F8VWF ELISA Kit, VWD ELISA Kit, coagulation factor VIII VWF
ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat von willebrand factor,vWF concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWF. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for vWF and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of vWF in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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