Enhanced ECL Western Blotting Substrate Kit

$150.00

  • Model: AS26-50
  • 50 Units in Stock
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Enhanced ECL Western Blotting Substrate Kit

Catalogue Number: AS26-50(50 tests)

                                   

Enhanced-ECL is a two-components product for the detection of horseradish peroxidase. This substrate is a highly sensitive, luminol-based solution that can be used for any blotting applications that utilize horseradish peroxidase (HRP)-conjugates, with minimal background interference.

REAGENTS:  50 Tests

Reagents A

luminol  2×concentrated solution

25ml

4-8℃

Reagents B

H2O2   2×concentrated solution

25ml

4-8℃

 

REAGENTS: 100 Tests

Reagents A

luminol  2×concentrated solution

50ml

4-8℃

Reagents B

H2O2   2×concentrated solution

50ml

4-8℃

 

REAGENTS: 200 Tests

Reagents A

luminol  2×concentrated solution

100ml

4-8℃

Reagents B

H2O2   2×concentrated solution

100ml

4-8℃

 

STORAGE:

• Store reagents in original bottles at the required conditions (one year)

• Avoid storing reagents or working solution in strong direct light.

 

PREPARATION OF SUBSTRATE WORKING SOLUTION:

  1. Mix Detection Reagents A & B at a 1:1 ratio.
  2. Immediately add the mixture to the blot. Use 0.125 ml working solution per cm2 of membrane.
  3. Incubate the blot for about 30-60 seconds at room temperature.
  4. Drain excess reagents.
  5. Cover blot with a clear plastic sheet protector or clear plastic wrap.
  6. Expose blot to X-ray film.

Test Data:

Enhanced ECL Western Blotting Substrate Kit

NOTES: Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

 

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