AD494 (DE3) Escherichia coli Strains

Alternative name      AD494 (DE3) Escherichia coli Strains,AD494(DE3),Escherichia coli
Packing 100ul             Storage at -20DEG C
Strains Resistance    Kan
Culture Medium       LB
Condition                37DEG C ,under the aerobic conditions

Resistance: Kan
Culture medium: LB
Strain category: Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Plasmids Transformation: heat shock at 42℃
Preservation method: 20% glycerin, -20℃
Basic application: for protein expression

AD494 (DE3) Escherichia coli Strains  Genotype 

D (ara-leu)7967, lacX74, phoAPvuII, phoR, malF3, trxB::Kan

AD494 (DE3) Escherichia coli Strains Description

AD494 strain is derived from K12 bacteria. AD494 strain contains thioredoxin reductase (TrxB) gene mutation, which is conducive to the formation of two sulfur bonds in bacterial cells, which helps proteins form the correct three-dimensional folding and form functional protein. The thioredoxin reductase (TrxB) mutation in AD494 strain has kanamycin resistance, so the host bacterium can only be used for ampicillin or other non kanamycin resistant plasmids, and for gene protein expression.