BL21 CodonPlus (DE3) -RIPL Escherichia coli Strains

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  • Model: S0006
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BL21 CodonPlus (DE3) -RIPL Escherichia coli Strains

Alternative name      BL21 CodonPlus (DE3) -RIPL Escherichia coli Strains,BL21 CodonPlus (DE3)-RIPL,Escherichia coli
Packing 100ul             Storage at -20DEG C
Strains Resistance    Tet/Chl/Spe/Str
Culture Medium       LB
Condition                37DEG C ,under the aerobic conditions

 

BL21 CodonPlus (DE3) -RIPL Escherichia coli Strains Genotype

F–ompT hsdS(rB– mB–) dcm+ Tetr galλ(DE3) endA Hte [argU proL Camr] [argU ileY leuW Strep/Specr]

 

BL21 CodonPlus (DE3) -RIPL Escherichia coli Strains information

esistance: Tet/Chl/Spe/Str

Culture medium: LB

Strain category: Escherichia coli

Culture conditions: 37 centigrade, aerobic, LB

Plasmids Transformation: heat shock at 42

Preservation method: 20% glycerin, -20 C

Basic application: for protein expression

 

BL21 CodonPlus (DE3) -RIPL Escherichia coli Strains Description

BL21 - CodonPlus (DE3) - RIPL strain is a Escherichia coli strain that contains tetracycline, chloramphenicol, streptomycin, and splendin, which is derived from the BL21 Gold strain of Stratagene, the lack of Lon protease and OmpT protease, thus reducing the degradation of recombinant proteins and supplementation of the 4 rare codons of the deficiency of Escherichia coli ( AGA, AUA, CCC, CUA,) corresponding tRNA (argU, ileY, proL, leuW), improve the expression level of the exogenous gene, especially the eukaryotic gene, which is rich in AT or GC- in the prokaryotic system. The strain of the strain is integrated with the DE3 region of the lambda phage (DE3 region contains T7 phage RNA polymerase), which can simultaneously express T7 RNA polymerase and RNA polymerase of Escherichia coli, which can be used for the protein expression of pET, pGEX, pMAL and other plasmids.

*Optimization for high level expression of heterologous protein

*No additional procedures can be used to express recombinant genes encoding rare passwords.

*Contains extra gene copies encoding the rare tRNA codon of Escherichia coli.

BL21-CodonPlus(DE3)-RIPL cells contain a ColE1-compatible, pACYC-based plasmid containing extra copies of the argU and proL tRNA genes and a ColE1- and pACYC-compatible pSC101- based plasmid containing extra copies of the argU, ileY, and leuW tRNA genes(argU (AGA, AGG), ileY (AUA) , proL (CCC), leuW (CUA)

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