BL21 (DE3) Escherichia coli Strains

S0001               Packing 100ul

Store at -20°C

Genotype
F- ompT hsdS(rB-mB-)gal dcm(DE3)

BL21 (DE3) Escherichia coli Strains Information

Resistance: None

Medium: LB

Strain category: Escherichia coli

Culture condition: 37 centigrade/span>, aerobic, LB

Plasmid transformation: 42 centigrade/span> heat shock

Induction method: IPTG or lactose and its analogues

Storage method: 20% glycerin, - 20 centigrade/span>

Basic application: for protein expression

Note: the most commonly used E.coli Expression bacteria, used in combination with PET series vectors
 

BL21 (DE3) Escherichia coli Strains Description

BL21 (DE3) is an E.coli Expression strain, which is a protein expression host with T7 RNA polymerase as the expression system. The expression of T7 phage RNA polymerase gene is controlled by lacuv5 promoter in DE3 region of λ phage, which is integrated on the chromosome of BL21. The strain is suitable for the expression of nontoxic proteins.

Caution:

1. This product is FOR RESEARCH USE ONLY!