BL21trxB (DE3) Escherichia coli Strains

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  • Model: S0008
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BL21trxB (DE3) Escherichia coli Strains

Alternative name      BL21trxB (DE3) Escherichia coli Strains,BL21trxB(DE3),Escherichia coli
Packing 100ul             Storage at -20DEG C
Strains Resistance    Kan
Culture Medium       LB
Condition                37DEG C ,under the aerobic conditions

Resistance: Kan
Culture medium: LB
Strain category: Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Plasmids Transformation: heat shock at 42degreebr /> Preservation method: 20% glycerin, -20degreebr /> Basic application: for protein expression

 

BL21trxB (DE3) Escherichia coli Strains Genotype

F-ompT hsdSB(rB- mB-)gal dcm trxB15::kan(DE3)

 

BL21trxB (DE3) Escherichia coli Strains Description

BL21 Trxb (DE3) strain is a commonly used recombinant protein expressing Escherichia coli, which can be cultured at 37 C, LB and aerobic. The strain is beneficial to the formation of target protein two sulfur bond in the cytoplasm, and can effectively enhance the correct folding and expression of two sulfur chain proteins. The BL21 Trxb host has the same mutant of the thioredoxin reductase (thioredoxin reductase mutation, Trxb) mutant that is the same as the AD494 strain. The strain was derived from the BL21 strain. Because the host bacteria containing Trxb protein can effectively improve the formation of two sulfur bonds in the cytoplasm, they can improve the correct folding rate of the target protein. The host bacteria have kanamycin resistance, so this strain can only be used in the expression of ampicillin resistant plasmid. DE3 is a source of lambda DE3, so a copy of a chromosome with T7 RNA polymerase is carried under the lacUV5 promoter. The strain is suitable for pET series vector and other T7 promoter series.

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