HB2151 Escherichia coli Strains

$255.00

  • Model: S0122
  • 48 Units in Stock
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HB2151 Escherichia coli Strains

Alternative name      HB2151 Escherichia coli Strains,HB2151,Escherichia coli
Packing 300ul             Storage at -20DEG C
Strains Resistance    
Culture Medium       LB
Condition                37DEG C ,under the aerobic conditions

Genotype            K12 (lac-pro), ara, nalr, thi/F'[proAB, lacIq, lacZ M15].

 

Operation Suggestion

1. strains preserved in glycerol medium 20%, received after the strain, please pick bacteria in 2x YT-N tablet (2xYT medium containing 100 g/ml nalidixic acid) on the line, 37 DEG C after overnight incubation, again from single colony in the Minimal Medium plate line, 37 degrees can be used after overnight culture, in order to make the cilia fully expressed, conducive to phage infection;
2. the bacteria that grow on the Minimal Medium plate should not be used in the refrigerator for a long time.

3. Minimal Medium plate prepare method:
Prepare stocks of the following:

       1 M MgCl2•6H2O: Dissolve 20.33 g in distilled water to a final volume of 100 ml and autoclave.
       1 M CaCl2•2H2O: Dissolve 14.7 g in distilled water to a final volume of 100 ml and autoclave.
       1 M thiamine hydrochloride: Dissolve 33.73 g in distilled water to a final volume of 100 ml and sterilize using a 0.22 μm filter.
       20% glucose: Dissolve 20 g of D-(+)-glucose (anhydrous) in distilled water to a final volume of 100 ml and sterilize by filtration through a 0.2 μm filter. Do not autoclave.
       In a 500 ml bottle, dissolve 6 g of Na2HPO4 (dibasic), 3 g of KH2PO4(monobasic) and 1 g of NH4Cl in distilled water to a final volume of 500 ml. adjust the pH to 7.4 with NaOH.
       In a separate 1 liter bottle, add distilled water to 15 g of Bacto-agar to a final Volume of 500 ml. Autoclave both bottles simultaneously to sterilize. Cool both bottles to 50-60°C and combine.  Add1 ml of 1 M MgCl2•6H2O, 1 ml of 1 M CaCl2•2H2O, 1 ml of 1 M thiamine hydrochloride and 5 ml of 20% glucose. Pour plates immediately. 

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