SM10 λpir Escherichia coli Strains

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SM10λpir Escherichia coli Strains

Alternative name      SM10λpir Escherichia coli Strains,SM10λpir,Escherichia coli
Packing 100ul             Storage at -20centigradebr /> Strains Resistance    Kan Resistance, no combination transfer experiment.

Culture Medium       LB
Condition                37centigrade,under the aerobic conditions

 

SM10λpir Escherichia coli Strains Genotype

thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu

 

SM10λpir Escherichia coli Strains Description

Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereo.E. coli SM10 λpir strain contains the pir gene (lysogenized with λpir phage) and it is designed for cloning and propagation of plasmids with R6K origin of replication sequence (such as pUT mini-Tn5 vectors). The plasmids containing the R6K origin of replication require the R6K-spicific replication protein lpir gene for propagation. The genotype of the strain is KmR, thi-1, thr, leu, tonA, lacY, supE, recA::RP4-2-Tc::Mu, pir.
 

SM10λpir Escherichia coli Strains Reference

1. Biswas I., Mettlach J. (2019) Targeted Gene Replacement in Acinetobacter baumannii. In: Biswas I., Rather P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY

DOI   https://doi.org/10.1007/978-1-4939-9118-1_10
Publisher Name    Humana Press, New York, NY

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