Y187 Yeast Strains
S0057 100ul
Y187 Yeast Strains Information
Alternative name Y187 Yeast Strains,Y187,Yeast
Packing 100ul Storage at -20℃
Strains Resistance Kan
Culture Medium YPDA
Condition 28℃,under the aerobic conditions
Y187 Yeast Strains Genotype
MATα, ura3-52, his3-200, ade 2-101, trp 1-901, leu 2-3, 112, gal4Δ, met-, gal80Δ, URA3::GAL1UAS-GAL1TATA-lacZ, MEL1
Y187 Yeast Strains Description
Y187 strain is a GAL4 system yeast single hybrid and double hybrid experimental strain, MAT α Type A can directly transform plasmids or perform screening experiments with MATa yeast strains (Y2HGold, AH109, etc.) through matching operations. The Transformation marker is trp1, leu2, and the reporting basis is lacZ, MEL1. The Y187-GAL4 yeast hybrid system requires two plasmids to be used in combination: pGBKT7 and pGADT7. The screening marker for plasmid pGBKT7 is TRP1, which is used to express the fusion protein of DNA-BD (amino acids from the N-terminus 1-174 of yeast transcription factor GAL4) and the target protein (Bait); The screening marker for plasmid pGADT7 is LEU, which is used to express the fusion protein of AD (amino acids 768-881 at the C-terminus of GAL4) and the target protein (Prey). The principle of GAL4 system: A complete yeast transcription factor GAL4 can be divided into two functionally independent domains: the DNA binding domain (DNA-BD) located in the N-terminus 1-174 amino acid region and the transcriptional activation domain (AD) located in the C-terminus 768-881 amino acid region. DNA-BD can recognize the upstream activation sequence UAS of GAL4 responsive gene and bind with it. AD can initiate transcription of genes downstream of UAS. BD and AD alone cannot activate transcription, but when they are close, they exhibit complete GAL4 activity, leading to transcriptional expression of downstream genes containing UAS promoters. Under normal conditions, BD does not bind to AD, and the protein to be detected fuses with BD and AD respectively, forming the bait fusion protein (bait BD) and prey fusion protein (prey AD). If there is an interaction between bait and prey, it will promote the proximity of BD and AD, forming a complete GAL4, thereby activating the transcription of the reporter gene. Y187 has two reporter genes: lacZ and MEL1, which are respectively initiated by two different promoters (G1 and M1). These two promoters only have the same 17 bp core region recognized by GAL4, while the rest are different, greatly reducing the probability of yeast double heterozygous false positives.
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. This product is glycerol packed, Please kindly culture it as soon as possible then harvest and store at -80℃.
3. Shipping temperature is 2-8℃.