AGL1 Agrobacterium Strain

$255.00

  • Model: STR3006
  • 18 Units in Stock
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AGL1 Agrobacterium Strain

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AGL1 Agrobacterium Strain Genotype  

C58 RecA (rif R/carbR) Ti pTiBo542DT-DNA (strepR) Succinamopine  

 

AGL1 Agrobacterium Strain Information

Type Agrobacterium tumefaciens

Resistance: Rif (20ug/ml)
Culture medium: YEB
Strain category: Agrobacterium tumefaciens
Culture conditions: 28 centigrade, aerobic, YEB
Transformation of plasmids: electric conversion and transformation
Preservation method: 30% glycerin, -20 C
Basic application: transgene

 

 

AGL1 Agrobacterium Strain Description

AGL1 strain was C58, RecA background, containing screening label, rifampicin resistant gene RIF and carbenicillin resistance gene carb in nuclear genes, in order to facilitate the transformation of the operation, the strains carrying Ti plasmid pTiBo542DT-DNA amber base without its transport function, the plasmid containing vir gene (VIR T-DNA gene was inserted into the plant genome required the T-DNA element, pTiBo542DT-DNA plasmid transfer function is damaged, but can help the binary vector T-DNA to successfully transfer). The pTiBo542DT-DNA Ti plasmid contains a screening tag: strep, giving AGL1 streptomycin resistance, which is suitable for transgenic operation of plants such as rice, Arabidopsis and poplar. AGL1 strain was cultured on LB or YEB at 28 centigrade aerobic culture, and 30% glycerol could be used to preserve the bacteria.

 

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