EHA105 Agrobacterium Strain


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EHA105 Agrobacterium Strain

Packing  100ul

Storage condition   -80 centigrade/p>


EHA105 Agrobacterium Strain Information

Resistance: Rif
Culture medium: YEB
Strain category: Agrobacterium tumefaciens
Culture conditions: 28 centigrade aerobic, YEB
Transformation of plasmids: electric conversion and transformation
Preservation method: 30% glycerin, -80centigradebr /> Basic application: transgene
Type Agrobacterium tumefaciens


EHA105 Agrobacterium Strain Description

The EHA105 strain by EHA101 strain and C58 type transformation, as the background, with screening label, rifampicin resistant gene RIF in nuclear genes, in order to facilitate the transformation of the operation, the strains carrying Ti plasmid pEHA105 amber base without its transport function (pTiBo542DT-DNA), the plasmid containing vir gene (VIR T-DNA gene was inserted into the plant genome the essential components of pEHA105 (pTiBo542DT-DNA) plasmid T-DNA transfer function itself is destroyed, but can be transferred to the binary vector T-DNA help smooth transfer). PEHA105 (pTiBo542DT-DNA) Ti plasmid contains screening Tags: strep, which endows EHA105 strains with streptomycin resistance, and is suitable for transgenic operation of plants such as rice and tobacco. The strain of EHA105 can be grown on YEB or LB medium. The optimum temperature is 28centigrade oxygen consumption, and 30% glycerol can be used to preserve the bacteria. The method of plasmids conversion is chemical conversion and electrical transformation.


EHA105 Agrobacterium Strain Genotype

C58 (rif R) Ti pEHA105 (pTiBo542DT-DNA) Succinamopine


EHA105 Agrobacterium Strain Reference

1.High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes
Tomasz Kowalczyk†, Aneta Gerszberg, Paulina Durańska, Róża Biłas and Katarzyna Hnatuszko-Konka†Email authorView ORCID ID profile
†Contributed equally
AMB Express 20188:125©  The Author(s) 2018
Received: 20 July 2018Accepted: 30 July 2018    Published: 6 August 2018


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