LBA4404 Agrobacterium Strain
Packing 300ul
Store 4 ℃
LBA4404 Agrobacterium Strain Genotype
Ach5 (RIF R) Ti pAL4404 (strepr) Octopine
LBA4404 Agrobacterium Strain Information
Resistance: Rif+Str
Culture medium: YEB
Strain category: Agrobacterium tumefaciens
Culture conditions: 28 centigrade, aerobic, YEB
Transformation of plasmids: electric conversion and transformation
Preservation method: 30% glycerin, -20 C
Basic application: transgene
Type Agrobacterium tumefaciens
LBA4404 Agrobacterium Strain Description
LBA4404 is Ach5 type background of Agrobacterium containing screening label, rifampicin resistant gene RIF in nuclear genes, in order to facilitate the transformation of the operation, the strains carrying octopine type Ti plasmid pAL4404 without its transport function, the plasmid containing vir gene (VIR T-DNA gene was inserted into the plant genome essential element, plasmid pAL4404 the T-DNA transfer function is damaged, but can be transferred to the binary vector T-DNA help smooth transfer). PAL4404 type Ti plasmid contains a screening label: strep, which endows streptomycin resistance to LBA4404 strain, and is suitable for transgenic plants such as tobacco, tomato, tobacco and other plants. The LBA4404 chemically transformed cells produced by our company were made by special technology, and the pCAMBIA2301 plasmid was used to detect the conversion efficiency of >104 cfu/ mu g DNA.
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