M15 (pREP4) Strains

$245.00

  • Model: STR0027
  • 100 Units in Stock
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M15 (pREP4) Strains

 

Catalog STR0027    Packing 100ul 
 

Alternative Name

M15 (pREP4) Strains,M15 Strains, M15 (pREP4) Strain

 

M15 (pREP4) Genotype 

M15[pREP4]genotype:derived from a strain K12, Nals strs rifs thi- lac- ara- gal+ mtl- F- recA+ uvr+ ion+ [pREP4 KanR]

 

M15 (pREP4) Information

Resistance: Kan 
Medium: LB 
Strain: E.Coli 
Culture conditions: 37centigradespan style="font-family: "times new roman", times, serif; font-size: 14px;">, aerobic, LB 
Plasmid transformation: 42centigradespan style="font-family: "times new roman", times, serif; font-size: 14px;"> heat shock 
Storage: 20% glycerin at -20
centigradebr /> Applications: For pQE vectors protein expression
More information: Expression under induction of IPTG

 

M15 (pREP4) Description

M15 strain contains a recombinant plasmid Prp4 containing a trans lac inhibitor to ensure highly regulated expression. Strain M15 [prep4] carried an inhibitory plasmid, prep4, which expressed lac inhibitor at a high level and trans inhibited the protein expression before IPTG induction. The stability of the plasmid was maintained by kanamycin resistance screening. M15 was successfully transferred into M15 by LB at 37 centigradeand heat shock at 42 centigrade In general, IPTG could make the strain express protein efficiently.

 

Caution:
Product is for research use only!


 

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