Technical data- Plasmid|ELISA kits| Antibodies|

Technical data


Question 1 :【How to handle the plasmid】 

A: The item is lyophilized form,  Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid. 

Question 2:【Plasmid transformation steps---E.coli】 

A: Operation steps:

The item is lyophilized form,  Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.

Transformation steps:
1)Take corresponding competent cells out from the refrigerator at – 80℃, thawed on the ice box at room temperature then labeled it;

2) Add 2μl plasmid into the competent cells and placed at 4℃for 30 min;

3. Heat shock in a 42℃ water bath for 1 min, and then place on the ice box for 2 min immediately ;

4. Added 900μl LB medium in each tube, shaking at 37℃, 150 rpm × 45 min;

5. Add 100 μl conversion solution into the corresponding resistant plate, pour the glass beads, and spread the liquid evenly; [2]

6. Invert the plate in constant temperature incubator at 37℃for overnight.

[1] The sterile centrifugal tube can be used in the sensory state.
[2] According to your request-Step 5, Centrifuge the conversion solution at 6000rpm for 3min, then leave 100 μ l of supernatant, mix and precipitate, and add all into the corresponding plate.
[3]Transformation should be accomplised in the ultra clean worktable.


【1】 How to drawing ELISA standard curve (ELISA KITS Result)

Method for drawing Standard curve


Drawing the ELISA standard curve by using specialized tools for curve fitting,

In this part, we will use the Curve Expert 1.3 for example:

Download link: Curve Expert 1.3


Please follow up the steps as below,


  1. Start "Curve Expert1.3"

2. X-axis: input values corresponding to the concentration, Y-axis: input the OD values of standard, as follow:


3.Click [Run], you will get this:



4 Click [ok], you will get two pictures as below,


5. A list of some curves appears at the upper right corner, from "1" Start to click the name of curves, then you will get  the corresponding fitting curve in the lower right corner.

6. Double click the best fitting curve for ELISA test, You will get the picture as below

Note: select curve equation with the best coefficient (the "r" value) for computing.The "r" value at the upper right corner (close to 1 means the better fitting)


7.  Then press [Ctrl] + [L] :



8. Enter the standard OD values, click [Calculate] , you will get the actual content of the target protein. (N-fold diluted samples, calculate the value should be multiplied by N).


9. If you would like to have the curve fitting equation of ELISA, right-click blank space of the dialog box in step 6, choose "Information".


10.Get the following, then click "Copy"

You can paste in the location you want to obtain the data as follows:

3rd degree Polynomial Fit:  y=a+bx+cx^2+dx^3...

Coefficient Data:

a =   -0.0010925264

b =  0.11260362

c =   0.00071609266

d =  -4.6172104e-005


【2】 MCP(monocyte chemoattractant protein-1)单核细胞趋化蛋白-1

MCP(monocyte chemoattractant protein-1)单核细胞趋化蛋白-1  Gene name: MCP,MCP-1/MCAF
Monocyte chemoattractant protein-1 (monocyte chemotactic protein 1,MCP-1), also known as monocyte chemoattractant and activator (monoctye chemotactic and activating factor,MCAF), belonging to the C-C subtribe . MCP-1/MCAF was originally from human myeloid monocytic line THP-1 and glioma cell lines as well as LPS or PHA stimulation found in human PBMC culture supernatant.

Body and body outside experiment are confirmed that MCP-1 on single nuclear cell has more activity, activated single nuclear cell and giant bite cell, makes its cell pulp within Ca2+ concentration increased, Super oxygen of produced and release, and release dissolved bacteria enzyme, raised single nuclear cell and giant bite cell stick attached molecular as Integrin family Beta 2 group and alpha 4 Molecular of expression and cell factor IL-1, and IL-6 of produced, activation of giant bite cell can inhibit tumor cell of growth. MCP is the Basophilic granulocyte chemotactic agent and activator, especially stimulating basophil degranulation and histamine-releasing more strongly. Combining the MCP-1 and MCP-1R-specific, this receptor is primarily distributed in mononuclear cells and myeloid progenitor cells and basophils, which belongs to the IL-8R family. Distribution of the Duffy Antigen on the surface of human red blood cells can also be combined with MCP-1.

In serum and synovial fluid of patients with rheumatoid arthritis MCP-1 levels increased significantly in synovial tissue composed of macrophages to produce MCP-1, MCP-1 associated with the pathology of rheumatoid arthritis. When high cholesterol and arterial medial smooth muscle cells to produce high levels of MCP-1, monocyte adhesion to the infiltration of blood vessels and arteries. Kidney inflammation, IL-1-induced Glomerular cell MCP-1. When Hyperlipidemia, LDL and Glomerular cells and stimulate them to produce MCP-1, Chemokine monocyte in the infiltration.

【3】Tumour ELISA KIT List for Lab Research 癌症研究相关 ELISA kit

We made a list of Tumour ELISA KIT for Lab Research (癌症研究相关 ELISA kit), click for download.


【4】ELISA KIT List for Diabetes Research   糖尿病研究相关 ELISA KIT

We made a ELISA KIT List for Diabetes Research (糖尿病研究相关 ELISA KIT),Click for download.


【5】Citation of  E0597p Porcine Malondialdehyde, MDA ELISA Kit :

Remote Ischemic Preconditioning May Attenuate Renal Ischemia-Reperfusion Injury in a Porcine Model of Supraceliac Aortic Cross-Clamping
Athanasiadis D.b · Kapelouzou A.a · Martikos G.b · Katsimpoulas M.a · Schizas D.b · Vasdekis S.N.c · Kostakis A.a · Liakakos T.D.d · Lazaris A.M.c

From:Vascular research journals  Vol. 52, No. 3, 2015 Issue release date: January 2016


【6】ELISA KIT List for Neuro biological research

We made a list of Neuro biological ELISA KIT for Lab Research , click for download.


【7】Related Pathological Research of Detecting Methylmalonic acid, Homocysteine and Vitamin B12 level By ELISA kit

Methylmalonic acid, also called MMA, is formed by the breakdown of Methylmalonyl CoA. We could detect the MMA in the urine samples if the patient has the problem of Methylmalonyl CoA metabolism by lack vitamin B12 .

Causes of Vitamin B12 deficiency

1.Decreased intake by losing weight or diabetes

2. Stomach and small bowel disorders and related surgery

3. pernicious anemia by Autoimmune

4.Medication interaction (e.g. Metformin, medications that reduce stomach acidity)

MMA concentrations in healthy people’ blood samples are between 73-271 nmol/L.So we could use MMA ELISA KIT quantitative determination of the MMA concentrations in plasma and urine.

New research shows that 25-20% of patients over the age of 70 have high levels of MMA, but they do not have B12 deficiency. For this reason, MMA test is not recommended in the people over the age of 70.

So we could use VB12 ELISA KIT and Hcy ELISA KIT quantitative determination of the VB12 , Hcy concentrations in plasma and urine.   Copyright by Nova Lifetech Inc.


【8】 Learn More About Elisa Kit Manufacturers 

Over the last few decades, there has been an enormous expansion of research focused on defining the role of inflammation in aging, age related diseases, disability, and frailty. The availability of methods to measure cytokines and other inflammatory mediators or markers with high sensitivity and specificity is critically important. Enzyme-Linked Immuno-Sorbant Assay (ELISA), the most widely used and best validated methods is limited by its ability to measure only a single protein in each sample. Thus, ELISA has been utilized as a diagnostic tool in plant pathology and medicine. Now, ELISA kit manufacturers have produced test kits that are used for cancer, autoimmune diseases, fertility, thyroid, steroid, infectious diseases and Parasitology, allergy, and diabetes tests.

One of test kits for cancer is HIF3-alpha-1 (HIF3A) ELISA KIT, which is used to increase the interaction transcriptional activity of HIF3A through increase stability. It is specially designed to function as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitates metabolic adaptation to hypoxia. HIF3A ELISA Kit plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Well, Human Nitric Oxide Synthase (NOS1) ELISA kit  is also a test kit for cancer. It is specially designed to Produce nitric oxide (NO), which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO display many properties of a neurotransmitter. Probably it has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Another example of test kit is the Human Nitric oxide synthase, endothelial (NOS3) ELISA KIT, which is used to produce nitric oxide (NO) that is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) - induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. The Aminopeptidase Q (APQ), another type of test kit is used for metalloprotease which is important for placentation by regulating biological activity of key peptides at the embryo maternal interface.

However, ELISA kit manufacturers also produce different type of test kits for infectious diseases as well. Human Tryptase, TPS ELISA KIT is FOR the major neutral protease present in mast cells and is secreted upon the coupled activation degranulation response of this cell type.

Human Serum amyloid A-4 protein (SAA4) ELISA KIT is FOR a major acute phase reactant. Apolipoprotein of the HDL complex. 


【9】Betatrophin for the diabetes

GM6484 gene encoding the Betatrophin protein , can significantly promote the rapid increase in the number of rat islet beta cells, by improving the expression of insulin, so as to achieve the purpose of treatment of diabetes. Betatrophin is a secreted protein, which is expressed in the liver, white fat and brown adipose tissue of mice, and can also be expressed in the liver tissues of the human body.


【10】Disarmed Agrobacterium tumefaciens strains defined by the Agrobacterium chromosomal background and Ti plasmid they harbor

Related chemically Competent Cells:

LBA4404 chemically Agrobacterium Express Competent Cells

GV3101 chemically Agrobacterium Express Competent Cells

EHA105 chemically Agrobacterium Express Competent Cells

AGL1 chemically Agrobacterium Express Competent Cells


11】 Amyotrophic lateral sclerosis ELISA KITS List

We made a list of Amyotrophic lateral sclerosis ELISA KITS,click for download.


12New progress in the study of diabetes


Asprosin arrived in the liver will lead to glucose excess production, which is an important feature of type II diabetes. If we offer asprosin antibody to the diabetic patients will let them fall blood sugar level, this would mean that patients can reduce or completely stop using medicine.

Now we could offer the Asprosin ELISA KIT and anti-Asprosin antibodies, Asprosin protein for lab research. 


13】Nova lifetech---- Strains List


14Nova lifetech---- Competent Cells List


15Transformation of Agrobacterium tumefaciens by freeze thawing method


(1) Add 1µl plasmid DNA  add to the 200ul Agrobacterium competent cells which thawing on the ice, and mixes it with an ice bath 30min.


(2) Quick freezing 5min in liquid nitrogen, 5min water bath at 37 DEG C, and rapid ice bath 2min.


(3) Add 800ul YEB liquid medium, 28 degrees rocked the 4 to 6hr.


(4) Take 50-200ul bacteria were coated on the YEB plate (containing the corresponding antibiotics) and cultured in 28 days for 2 days.


Related Agrobacterium products

A4 chemically Agrobacterium Express Competent Cells

AGL1 chemically Agrobacterium Express Competent Cells

AGL1 Electro Agrobacterium Express Competent Cells

Ar A4 chemically Agrobacterium Express Competent Cells

Ar qual chemically Agrobacterium Express Competent Cells

Ar.A4 Electro Agrobacterium Express Competent Cells

Ar.Qual Electro Agrobacterium Express Competent Cells

EHA101 chemically Agrobacterium Express Competent Cells

EHA101 Electro Agrobacterium Express Competent Cells

EHA105 chemically Agrobacterium Express Competent Cells

EHA105 Electro Agrobacterium Express Competent Cells

EHA106 chemically Agrobacterium Express Competent Cells

GV3101 chemically Agrobacterium Express Competent Cells

GV3101 Electro Agrobacterium Express Competent Cells

If you have any further inquiry please contact us:

Copyright Nova lifetech Inc.  


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