Rat Hyaluronan and proteoglycan link protein 1, HAPLN1 ELISA KIT ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HAPLN1,CRTL1; Cartilage link protein; cartilage linking protein 1; cartilage-link protein; cartilage-linking protein 1; proteoglycan link protein; hyaluronan and proteoglycan link protein 1

Search name

Rat HAPLN1 ELISA KIT ,Rat CRTL1 ELISA KIT ,Rat Cartilage link protein ELISA KIT ,Rat cartilage linking protein 1 ELISA KIT ,Rat cartilage-link protein ELISA KIT ,Rat cartilage-linking protein 1 ELISA KIT ,Rat proteoglycan link protein ELISA KIT ,Rat hyaluronan and proteoglycan link protein 1 ELISA KIT

Intended use

Rat HAPLN1 ELISA KIT allows for the in vitro quantitative determination of Rat Hyaluronan and proteoglycan link protein 1, HAPLN1 ELISA KIT concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Rat HAPLN1 ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in Rat HAPLN1 ELISA KIT has been pre-coated with an antibody specific to HAPLN1, During the reaction, HAPLN1 in the sample or standard competes with a fixed amount of biotin-labeled HAPLN1 for sites on a pre-coated Monoclonal antibody specific to HAPLN1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HAPLN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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