Bovine Tacrolimus, FK-506 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Tacrolimus,fujimycin, FK-506, fujimycin, Prograf, Advagraf, Protopic
Search name
Bovine Tacrolimus ELISA Kit, Bovine fujimycin ELISA Kit, Bovine FK-506 ELISA Kit, Bovine Prograf ELISA Kit, Bovine Advagraf ELISA Kit, Bovine Protopic ELISA Kit
Intended use
Bovine FK-506 ELISA Kit allows for the in vitro quantitative determination of Bovine tacrolimus,Tacrolimus concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine FK-506 ELISA Kit has been pre-coated with an antibody specific to Tacrolimus. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tacrolimus and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tacrolimus, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tacrolimus in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine FK-506 ELISA Kit PDF manual download
Bovine FK-506 ELISA Kit Range 0.15-10ng/ml
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