Rat Tacrolimus, FK-506 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Tacrolimus,fujimycin, FK-506, fujimycin, Prograf, Advagraf, Protopic
Search name
Rat Tacrolimus ELISA Kit, Rat fujimycin ELISA Kit, Rat FK-506 ELISA Kit, Rat Prograf ELISA Kit, Rat Advagraf ELISA Kit, Rat Protopic ELISA Kit
Intended use
Rat FK-506 ELISA Kit allows for the in vitro quantitative determination of Rat tacrolimus,Tacrolimus concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Tacrolimus ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in Tacrolimus ELISA KIT has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectro- photometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat FK-506 ELISA Kit PDF manual download
Rat FK-506 ELISA Kit Range 1.56-100ng/ml
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