A4 Agrobacterium Strain
STR3001 Packing 100ul
Store at -80℃
A4 Agrobacterium Strain Information
Resistance: Kan
Culture medium: TY
Strain category: Agrobacterium tumefaciens
Culture conditions: 30℃, aerobic, TY
Preservation method: 20% glycerin, -80℃
Basic application: Transgene
A4 Agrobacterium Strain Genotype
Agrobacterium rhizogenes(kanR) Ar A4 Ri(agropine type)
A4 Agrobacterium Strain Description
Type: Agrobacterium rhizogenes
Resistance: Kan
Growth Condition: LB/ 30℃
A4 Agrobacterium tumefaciens is a natural Agrobacterium alkaline strain. It has kanamycin resistance and can be cultured on YT medium. The optimal temperature for culture is 30 ℃.
Operating instructions:
1. This product contains a portion of glycerin bacteria. When using this glycerin bacteria, you can dip a small amount of coated plate or liquid culture on the surface of glycerin bacteria without complete melting. It can also be used after complete thawing, but with the increase of freeze-thaw times, the activity of bacteria will gradually decline.
2. In order to ensure the purity of bacteria and avoid other bacterial contamination, we should first draw the plate, and then select the monoclone colony for subsequent operation.
Antibiotics for Agrobacterium
| Antibiotics | Method | Stock solution | fluid |
| Carb | Dissolved in DDW(Double distilled water), filtration sterilization by 0.22 m membrane filtration | 50mg/ml | 50ug/ml |
| Kan | Dissolved in DDW(Double distilled water), filtration sterilization by 0.22 m membrane filtration | 50mg/ml | 50ug/ml |
| Strep | Dissolved in DDW(Double distilled water), filtration sterilization by 0.22 m membrane filtration | 10mg/ml | 50ug/ml |
| Rif | Dissolved in DMSO, filtration sterilization by 0.22 m membrane filtration | 10mg/ml | 20ug/ml |
| gent | Dissolved in DDW(Double distilled water), filtration sterilization by 0.22 m membrane filtration | 20mg/ml | 40ug/ml |
LB and YEB
| Component | LB (Liquid)/L | LB( Solid /L | Component | YEB (Liquid)/L | YEB ( Solid /L |
| Tryptone | 10g | 10g | Tryptone | 5g | 5g |
| Yeast extract | 5g | 5g | Yeast extract | 1g | 1g |
| NaCl | 10g | 10g | Beef extract | 5g | 5g |
| NaOH | PH TO 7 | PH TO 7 | Sucrose | 5g | 5g |
| Agar | - | 15g | MgSO4*7H2O | 0.49g | 0.49g |
| | | | NaOH | PH TO 7 | PH TO 7 |
| | | | Agar | - | 15g |
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. This product is glycerol packed, Please kindly culture it as soon as possible then harvest and store at -80℃.
3. Shipping temperature is 2-8℃.
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