Bovine CCK ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Bovine CCK ELISA KIT ,Bovine Cholecystokinin ELISA KIT ,Bovine prepro-cholecystokinin ELISA KIT ,Bovine cholecystokinin triacontatriapeptide ELISA KIT
Intended use
Bovine CCK ELISA kit allows for the in vitro quantitative determination of Bovine CCK concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Bovine CCK ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine CCK ELISA kit Range 46.8-3000g/ml
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