Canine intercellular adhesion molecule 1 ELISA Kit
Product Name:Canine intercellular adhesion molecule 1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0048d
Gene Name:Canine intercellular adhesion molecule 1
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine intercellular adhesion molecule 1
Alternative Name:intercellular adhesion molecule 1,intercellular adhesion molecule 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine intercellular adhesion molecule 1 ELISA Kit allows for the in vitro quantitative determination of Canine intercellular adhesion molecule 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine intercellular adhesion molecule 1 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Canine intercellular adhesion molecule 1 ELISA KIT ,Canine ICAM-1 ELISA KIT ,Canine CD54 ELISA KIT ,Canine canine rhinovirus receptor ELISA KIT ,Canine intercellular adhesion molecule 1 ELISA KIT ,Canine BB2 ELISA KIT ,Canine CD54 ELISA KIT ,Canine P3.58 ELISA KIT ,Canine cell surface glycoprotein P3.58 ELISA KIT ,Canine major group rhinovirus receptor ELISA KIT
Intended use
Canine intercellular adhesion molecule 1 ELISA kit allows for the in vitro quantitative determination of Canine intercellular adhesion molecule 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Canine intercellular adhesion molecule 1 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for intercellular adhesion molecule 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain intercellular adhesion molecule 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of intercellular adhesion molecule 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Canine intercellular adhesion molecule 1 ELISA kit Range 0.15-10ng/ml
intercellular adhesion molecule 1 Related Products
> intercellular adhesion molecule 1 ELISA KITS
> intercellular adhesion molecule 1 Recombinant Protein
> intercellular adhesion molecule 1 Antidodies
has been pre-coated with an Canine intercellular adhesion molecule 1 antibody specific to Canine intercellular adhesion molecule 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine intercellular adhesion molecule 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine intercellular adhesion molecule 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine intercellular adhesion molecule 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!