Human Angiotensin IV, ANGIV ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Human ANGIV ELISA Kit, Human Angiotensin IV ELISA Kit, Human ANG-IV ELISA Kit, Human ANG IV ELISA Kit
Intended use
Human ANGIV ELISA kit allows for the in vitro quantitative determination of Human Angiotensin IV concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Human ANGIV ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANGIV, During the reaction, ANGIV in the sample or standard competes with a fixed amount of biotin-labeled ANGIV for sites on a pre-coated Monoclonal antibody specific to ANGIV. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANGIV in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human ANGIV ELISA kit Range 0.15-10ng/ml
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