Rat Peroxiredoxin-1, PRDX1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Peroxiredoxin-1,Thioredoxin-dependent peroxide reductase 2,Thioredoxin peroxidase 2,Natural killer cell-enhancing factor A,NKEF-A,Proliferation-associated gene protein,PAG, PRDX1,SP23; NKEF-A; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2; natural killer cell-enhancing factor A; natural killer-enhancing factor A; peroxiredoxin-1; proliferation-associated gene A; proliferation-associated gene protein; thioredoxin peroxidase 2; thioredoxin-dependent peroxide reductase 2; peroxiredoxin 1, Macrophage 23 kDa stress protein,Osteoblast-specific factor 3,OSF-3
Search name
Rat Peroxiredoxin-1 ELISA KIT,Rat Thioredoxin-dependent peroxide reductase 2 ELISA KIT,Rat Thioredoxin peroxidase 2 ELISA KIT,Rat Natural killer cell-enhancing factor A ELISA KIT,Rat NKEF-A ELISA KIT,Rat Proliferation-associated gene protein ELISA KIT,Rat PAG ELISA KIT,Rat PRDX1 ELISA KIT,Rat SP23 ELISA KIT,Rat NKEF-A ELISA KIT,Rat NKEFA ELISA KIT,Rat PAG ELISA KIT,Rat PAGA ELISA KIT,Rat PAGB ELISA KIT,Rat PRX1 ELISA KIT,Rat PRXI ELISA KIT,Rat TDPX2 ELISA KIT,Rat natural killer cell-enhancing factor A ELISA KIT,Rat natural killer-enhancing factor A ELISA KIT,Rat peroxiredoxin-1 ELISA KIT,Rat proliferation-associated gene A ELISA KIT,Rat proliferation-associated gene protein ELISA KIT,Rat thioredoxin peroxidase 2 ELISA KIT,Rat thioredoxin-dependent peroxide reductase 2 ELISA KIT,Rat peroxiredoxin 1 ELISA KIT,Rat Macrophage 23 kDa stress protein ELISA KIT,Rat Osteoblast-specific factor 3 ELISA KIT,Rat OSF-3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Peroxiredoxin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Peroxiredoxin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Peroxiredoxin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Peroxiredoxin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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